Protocols: Amplification
The amplification of target DNA is an important stage in current sequencing methods. DNA can be replicated using bacterial cultures (cloning), or via the polymerase chain reaction. PCR is often preferred due to the speed and high through-put of PCR systems. This is accomplished by placing the DNA extractions in a specialized chemical environment and cycling them through a specific temperature regime. A heat-activated DNA polymerase replicates the target DNA sequence with each cycle.
A critical component of the amplification process is the selection of appropriate primers.
Protocols
PCR reagents, thermocycling profiles, and general practices are available in PDF format here.
Primer sets comprising major analytical pipelines at CCDB are available in PDF format here.